Live Cell Super-Resolution Microscopy
July 23rd, 2019 STEFAN WIESER
ICFO-The Institute of Photonic Sciences

Cell Theory of life claims that the basic unit of any living matter is a cell. Cells are very complex machineries made of billions of components like proteins and lipids, constantly operating at reduced entropy and inherently playing with high variability and stochasticity. To understand life, we need a way to observe the molecular details operating in space and time in a cell. However, using a classical microscope, the diffraction limit of light limits the observation to around 200nm and illumination of the samples with high laser power bleaches fluorescent labeled cell structures and proteins and induces phototoxicity.

I this lecture I will explain how live cell Superresolution microscopy can help to unravel molecular details down to 1nm spatial resolution at highest time resolution with some hundreds of photons.

I will describe the limits of state-of-the-art super resolution microscopy on living cells and explain what structured illumination microscopy can do to help unraveling morphogenetic movements and immune cell migration of embryonic stem cells and immune cells.

Tuesday, July 23, 2019, 12:00. ICFO’s Blue Lecture Room