Light Seminars
September 27, 2013
L4H SEMINAR SEBASTIAN DEINDL 'A novel nucleosome remodeling mechanism revealed by single-molecule fluorescence microscopy'
L4H SEMINAR SEBASTIAN DEINDL 'A novel nucleosome remodeling mechanism revealed by single-molecule fluorescence microscopy'
SEBASTIAN DEINDL
Friday, September 27, 2013, 12:00. Seminar Room
SEBASTIAN DEINDL
Department of Physics, Harvard University
Cambridge, Massachusetts, USA
SEBASTIAN DEINDL
Department of Physics, Harvard University
Cambridge, Massachusetts, USA
ISWI-family enzymes remodel chromatin (the packaged state of DNA) by sliding nucleosomes along DNA. Nucleosomes are the fundamental repeating units of chromatin, and the mechanism by which remodeling enzymes can shift the position of nucleosomes on the DNA remains unclear. We have applied single-molecule fluorescence imaging approaches to visualize nucleosome sliding by ISWI-family remodelers in real-time. They translocate nucleosomes with a conserved stepping pattern that is maintained by the catalytic subunit of the remodelers. Our results suggest a new model for nucleosome remodeling: translocation proceeds in well-defined multi-bp entry steps and 1 bp exit steps and DNA translocation to the exit side precedes any DNA movement on the entry side.
Friday, September 27, 2013, 12:00. Seminar Room
Hosted by Prof. Melike Lakadamyali
Friday, September 27, 2013, 12:00. Seminar Room
Hosted by Prof. Melike Lakadamyali
Light Seminars
September 27, 2013
L4H SEMINAR SEBASTIAN DEINDL 'A novel nucleosome remodeling mechanism revealed by single-molecule fluorescence microscopy'
L4H SEMINAR SEBASTIAN DEINDL 'A novel nucleosome remodeling mechanism revealed by single-molecule fluorescence microscopy'
SEBASTIAN DEINDL
Friday, September 27, 2013, 12:00. Seminar Room
SEBASTIAN DEINDL
Department of Physics, Harvard University
Cambridge, Massachusetts, USA
SEBASTIAN DEINDL
Department of Physics, Harvard University
Cambridge, Massachusetts, USA
ISWI-family enzymes remodel chromatin (the packaged state of DNA) by sliding nucleosomes along DNA. Nucleosomes are the fundamental repeating units of chromatin, and the mechanism by which remodeling enzymes can shift the position of nucleosomes on the DNA remains unclear. We have applied single-molecule fluorescence imaging approaches to visualize nucleosome sliding by ISWI-family remodelers in real-time. They translocate nucleosomes with a conserved stepping pattern that is maintained by the catalytic subunit of the remodelers. Our results suggest a new model for nucleosome remodeling: translocation proceeds in well-defined multi-bp entry steps and 1 bp exit steps and DNA translocation to the exit side precedes any DNA movement on the entry side.
Friday, September 27, 2013, 12:00. Seminar Room
Hosted by Prof. Melike Lakadamyali
Friday, September 27, 2013, 12:00. Seminar Room
Hosted by Prof. Melike Lakadamyali
All Insight Seminars
Light Seminars
December 11, 2013
L4H SEMINAR JEAN FRANCOIS LEGER 'Exploring the Functions of the Brain in Vivo with Two-Photon Microscopy: the Case of the Tactile Cortex of Rat'
Light Seminars
November 26, 2013
L4H SEMINAR KHALID SALAITA 'Using Light to Visualize Molecular Forces in Cells'
Light Seminars
November 14, 2013
L4H SEMINAR HATICE ALTUG 'Integrated Nanoplasmonic Systems for Ultrasensitive Spectroscopy and High-Throughput Bio-Detection'
Light Seminars
November 6, 2013
L4H Seminar MARIE-CLAIRE SCHANNE-KLEIN'In Situ Visulalization of Collagen Architecture in Biological Tissues Using Polarization-Resolved SHG Microscopy'
Light Seminars
October 30, 2013
L4H Seminar ALFRED J. MEIXNER 'Tip-Enhanced Nanometer Scale Optical Imaging And Spectroscopy'
Light Seminars
October 18, 2013
L4H SEMINAR DAVID RUEDA 'Watching AID/APOBEC3G Scanning Single Stranded and Transcribed DNA with Single Molecule Resolution'
Light Seminars
September 23, 2013
L4H SEMINAR VALENTINA EMILIANI 'Two-photon optogenetics by wave front shaping of ultrafast pulses'
Light Seminars
September 18, 2013
L4H SEMINAR TERESA NEVES PETERSEN 'Photonic cancer therapy: modulating cellular metabolism with light'
Light Seminars
September 4, 2013
L4H SEMINAR MIKE HEILEMANN 'Quantitative single-molecule super-resolution microscopy of cellular structures'
Light Seminars
July 3, 2013
L4H SEMINAR PAUL W. WISEMAN 'Mapping Adhesion, Cytoskeletal and Signaling Protein Transport and Interactions in Living Cells by Image Correlation Methods'
Light Seminars
June 12, 2013
L4H Seminar JONAS RIES 'Novel Labeling Schemes for Single-Molecule Localization Microscopy'
Light Seminars
May 29, 2013
Light Seminars
May 21, 2013
L4H Seminar XAVIER INTES 'Towards Whole-Body Foster Resonance Energy Transfer Pre-Clinical Imaging'
Light Seminars
April 22, 2013
L4H SEMINAR CORINNE LORENZO 'Development of 3D Imaging of Large Spheroid Tumor Models Using Light Sheet Microscopy'
Light Seminars
March 13, 2013
L4H Seminar JORGE RIPOLL 'From ballistic to diffusive regimes: Light Propagation Models and Applications for In-vivo Optical Tomography'
Light Seminars
February 20, 2013
L4H Seminar ANDREU LLOBERA 'Photonic Lab on a Chip: Mergence of Photonics and Microfluidics for Real Time Cell Screening'