The neocortex is a highly interconnected network of cells which is organized in multiple layers and which plays a fundamental role in brain functions such as sensory perception and the generation of motor commands. However, how these complex functions are generated by underlying network dynamics is still unclear. To decipher the computational principles of individual cells within the neocortical circuitry, new technologies to monitor and manipulate neuronal activity with improved spatial and temporal resolution are needed. In this presentation, we will focus on optical techniques based on structured light illumination by phase modulation with Liquid Crystal On Silicon Spatial Light Modulators (LCOS-SLMs). We will review current applications of this technology to the optical dissection of neuronal circuits in combination with light-sensitive dyes and proteins. More specifically, we will focus on the use of LCOS-SLMs and two-photon excitation for fast three-dimensional imaging of fluorescent cellular signals in the neocortex and for patterned photo-stimulation of caged-compounds and light-sensitive actuators.


Wednesday, December 14, 2011, 10:30. Blue Lecture Room

Hosted by Prof. Melike Lakadamyali