Light Seminars
July 11, 2011
L4H Seminar ALEKSANDRA RADENOVIC 'Photonics Tools for Single Molecule Biophysics'
L4H Seminar ALEKSANDRA RADENOVIC 'Photonics Tools for Single Molecule Biophysics'
ALEKSANDRA RADENOVIC
Seminar, July 11, 2011, 12:00. Seminar Room
ALEKSANDRA RADENOVIC
EPFL – STI/SV - IBI - LBEN, Lausanne, SWITZERLAND
ALEKSANDRA RADENOVIC
EPFL – STI/SV - IBI - LBEN, Lausanne, SWITZERLAND
In this talk I will focus on two research areas pursued in my laboratory: The use of novel fabricated or chemically synthesized nano-optomechanical for single molecule biophysics and on opportunities and challenges of using localization microscopy such as PALM or STORM in quantitative biology.
The paramount goal in the nano-biotechnology area is to develop highly biocompatible nanostructures for targeted imaging, mechanical stimulation, drug delivery and cancer therapy. The tools of nanotechnology and modern cell biology now provide the means to investigate many of the physical aspects of complex processes at the micro- and nanometer scale. In our lab we use nanowires or nanofabricated structures as mechano-optical probes for the study of nanoscale cellular components and as probes in the optical tweezers. Nanowires with controlled size and shape are synthesized either via wet chemical methods or using nanolithography approach. Synthesized nanowires are imaged with transmission electron microscope TEM and characterized optically to investigate what is an effect of the nanowire size, shape and material on second harmonic generation efficiency3 –namely their nonlinear optical conversion capability. In addition, we also need to render them biocompatible.
Super-resolution techniques based on the sequential photoswitching/photo-activation of single photo-emitters have allowed detection of single molecules with spatial localization accuracy below 10 nanometers and Nyquist-Shannon-limited resolution of approximately 20 nm. In the case of PALM, the key idea is that super-resolution images are constructed from rounds of photo-activating sparse subsets of a sample, allowing the localization of those single emitters with high precision, building up over time the complete image with high spatial resolution. Although several groups have reported super-resolution images of cellular structures in living or fixed cells, so far only a few studies used PALM/STORM data in a quantitative way.
Seminar, July 11, 2011, 12:00. Seminar Room
Hosted by Prof. Melike Lakadamyali
The paramount goal in the nano-biotechnology area is to develop highly biocompatible nanostructures for targeted imaging, mechanical stimulation, drug delivery and cancer therapy. The tools of nanotechnology and modern cell biology now provide the means to investigate many of the physical aspects of complex processes at the micro- and nanometer scale. In our lab we use nanowires or nanofabricated structures as mechano-optical probes for the study of nanoscale cellular components and as probes in the optical tweezers. Nanowires with controlled size and shape are synthesized either via wet chemical methods or using nanolithography approach. Synthesized nanowires are imaged with transmission electron microscope TEM and characterized optically to investigate what is an effect of the nanowire size, shape and material on second harmonic generation efficiency3 –namely their nonlinear optical conversion capability. In addition, we also need to render them biocompatible.
Super-resolution techniques based on the sequential photoswitching/photo-activation of single photo-emitters have allowed detection of single molecules with spatial localization accuracy below 10 nanometers and Nyquist-Shannon-limited resolution of approximately 20 nm. In the case of PALM, the key idea is that super-resolution images are constructed from rounds of photo-activating sparse subsets of a sample, allowing the localization of those single emitters with high precision, building up over time the complete image with high spatial resolution. Although several groups have reported super-resolution images of cellular structures in living or fixed cells, so far only a few studies used PALM/STORM data in a quantitative way.
Seminar, July 11, 2011, 12:00. Seminar Room
Hosted by Prof. Melike Lakadamyali
Light Seminars
July 11, 2011
L4H Seminar ALEKSANDRA RADENOVIC 'Photonics Tools for Single Molecule Biophysics'
L4H Seminar ALEKSANDRA RADENOVIC 'Photonics Tools for Single Molecule Biophysics'
ALEKSANDRA RADENOVIC
Seminar, July 11, 2011, 12:00. Seminar Room
ALEKSANDRA RADENOVIC
EPFL – STI/SV - IBI - LBEN, Lausanne, SWITZERLAND
ALEKSANDRA RADENOVIC
EPFL – STI/SV - IBI - LBEN, Lausanne, SWITZERLAND
In this talk I will focus on two research areas pursued in my laboratory: The use of novel fabricated or chemically synthesized nano-optomechanical for single molecule biophysics and on opportunities and challenges of using localization microscopy such as PALM or STORM in quantitative biology.
The paramount goal in the nano-biotechnology area is to develop highly biocompatible nanostructures for targeted imaging, mechanical stimulation, drug delivery and cancer therapy. The tools of nanotechnology and modern cell biology now provide the means to investigate many of the physical aspects of complex processes at the micro- and nanometer scale. In our lab we use nanowires or nanofabricated structures as mechano-optical probes for the study of nanoscale cellular components and as probes in the optical tweezers. Nanowires with controlled size and shape are synthesized either via wet chemical methods or using nanolithography approach. Synthesized nanowires are imaged with transmission electron microscope TEM and characterized optically to investigate what is an effect of the nanowire size, shape and material on second harmonic generation efficiency3 –namely their nonlinear optical conversion capability. In addition, we also need to render them biocompatible.
Super-resolution techniques based on the sequential photoswitching/photo-activation of single photo-emitters have allowed detection of single molecules with spatial localization accuracy below 10 nanometers and Nyquist-Shannon-limited resolution of approximately 20 nm. In the case of PALM, the key idea is that super-resolution images are constructed from rounds of photo-activating sparse subsets of a sample, allowing the localization of those single emitters with high precision, building up over time the complete image with high spatial resolution. Although several groups have reported super-resolution images of cellular structures in living or fixed cells, so far only a few studies used PALM/STORM data in a quantitative way.
Seminar, July 11, 2011, 12:00. Seminar Room
Hosted by Prof. Melike Lakadamyali
The paramount goal in the nano-biotechnology area is to develop highly biocompatible nanostructures for targeted imaging, mechanical stimulation, drug delivery and cancer therapy. The tools of nanotechnology and modern cell biology now provide the means to investigate many of the physical aspects of complex processes at the micro- and nanometer scale. In our lab we use nanowires or nanofabricated structures as mechano-optical probes for the study of nanoscale cellular components and as probes in the optical tweezers. Nanowires with controlled size and shape are synthesized either via wet chemical methods or using nanolithography approach. Synthesized nanowires are imaged with transmission electron microscope TEM and characterized optically to investigate what is an effect of the nanowire size, shape and material on second harmonic generation efficiency3 –namely their nonlinear optical conversion capability. In addition, we also need to render them biocompatible.
Super-resolution techniques based on the sequential photoswitching/photo-activation of single photo-emitters have allowed detection of single molecules with spatial localization accuracy below 10 nanometers and Nyquist-Shannon-limited resolution of approximately 20 nm. In the case of PALM, the key idea is that super-resolution images are constructed from rounds of photo-activating sparse subsets of a sample, allowing the localization of those single emitters with high precision, building up over time the complete image with high spatial resolution. Although several groups have reported super-resolution images of cellular structures in living or fixed cells, so far only a few studies used PALM/STORM data in a quantitative way.
Seminar, July 11, 2011, 12:00. Seminar Room
Hosted by Prof. Melike Lakadamyali
All Insight Seminars
Light Seminars
December 14, 2011
L4H Seminar TOMMASO FELLIN 'Optical Investigation of Neocortical Circuits with Structured Light Illumination by Phase Modulation'
Light Seminars
November 30, 2011
L4H Seminar EVA RITTWEGER 'Optical Microscopy with Nanoscale Resolution'
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November 4, 2011
L4H Seminar JOSEP DALMAU 'Syndromes and Mechanisms of Autoimmune Synaptic Encephalitides'
Light Seminars
November 2, 2011
L4H Seminar JOCHEN GUCK 'Dual-Beam Laser Traps in Biology and Medicine – When One Beam Is Not Enough'
Light Seminars
October 3, 2011
L4H Seminar* VASILIS NTZIACHRISTOS 'Illuminating Biomedical Discovery with Advanced Photonic Imaging'
Light Seminars
July 20, 2011
L4H Seminar JAN LAUFER 'In vivo small animal imaging using an all-optical photoacoustic scanner'
Light Seminars
July 6, 2011
L4H Seminar SANTIAGO NONELL 'Singlet Oxygen in Photobiology: from Basic Science to Applications in Photodynamic Therapy'
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June 28, 2011
L4H Seminar MIQUEL BOSCH 'The Persistence of Memory: Two-Photon Imaging Reveals how Synapses Learn and Remember in Real Time'
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June 22, 2011
L4H Seminar ANDREW DUNN 'Optical Imaging of Cerebral Blood Flow'
Light Seminars
May 18, 2011
L4H Seminar ANABELA DA SILVA 'In vivo optical molecular imaging closer to clinical'
Light Seminars
May 4, 2011
L4H Seminar HAMID DEHGHANI 'Of Mice and Men: Adventures in Optical Imaging'
Light Seminars
April 20, 2011
L4H Seminar SULIANA MANLEY 'Quantitative Static and Dynamic Imaging with Photoactivatable Fluorescence'
Light Seminars
April 13, 2011
L4H Seminar* TONY WILSON 'Making Light Work in Microscopy'
Light Seminars
April 6, 2011
L4H Seminar JORDI SORIANO FRADERA 'Experiments on Patterned Neuronal Networks. Probing Cultures to Understand Brain Complexity'
Light Seminars
March 23, 2011
L4H Seminar RICARDO ARIAS-GONZÁLEZ 'Unveiling Physics in Biology by Optical Manipulation of Single Molecules'
Light Seminars
March 21, 2011
L4H Seminar BRETT BOUMA 'Cancer Screening and Surveillance with Optical Coherence Tomography'
Light Seminars
March 9, 2011
L4H Seminar DOUGLAS WEIBEL 'Membrane Curvature Controls Lipid Microdomain Formation and Protein Localization in Bacteria'
Light Seminars
February 28, 2011
L4H Seminar* MARTIN B. van der MARK 'Diffuse optical spectroscopy with very high collection efficiency'
Light Seminars
February 23, 2011
L4H Seminar* ILIAS TACHTSIDIS 'Measuring Brain Tissue Energy Metabolism Using Light'
Light Seminars
February 9, 2011
L4H Seminar MARK BATES 'Single-Molecule Localization Methods for Super-Resolution Fluorescence Microscopy'
Light Seminars
January 26, 2011
L4H Seminar JAMES SHARPE 'Imaging the Dynamics of Morphogenesis: from Dynamic to Static, from Hi-Res to Low'