Hour: From 12:00h to 13:00h
Place: Online (Zoom)
L4H SEMINAR: Light Sheet Fluorescence Microscope with an Optofluidic chip
Abstract:
Opto-fluidic technologies integrate multiple fluidics and photonics devices in a miniaturized chip. Among many lab-on-chip applications, they can be used for optical microscopy [1].
The seminar will discuss the design and applications of a millimeter-scaled optofluidic device that incorporates light-sheet illumination and automatic sample delivery and scanning. This device upgrades a standard inverted microscope to a high throughput, three-dimensional, Light Sheet Fluorescence Microscope. A cylindrical lens fabricated by Femtosecond Laser Irradiation followed by Chemical Etching (FLICE) is integrated with an optical waveguide or an optical fiber on a fused silica chip. The lens is used to illuminate a single plane of the sample, at two alternating wavelengths. The samples flow in a three-dimensional fluidic network (fabricated by FLICE too) and is then imaged on a CMOS running at camera at 400kHz.
Several applications will be discussed in the seminar, together with the technological solutions for automatic sample alignment, including automatic imaging of tumor spheroids [2], Drosophila embryos, and high NA (1.1) imaging of cancer cells [3].
[1] Paiè, P., Martínez Vázquez, R., Osellame, R., Bragheri, F., and Bassi, A. “Microfluidic based optical microscopes on Chip” Cytometry Part A, 93, 987-996 (2018).
[2] Paiè, P., Bragheri, F., Bassi, A., and Osellame, R. “Selective plane illumination microscopy on a chip” Lab on a chip, 16(9), 1556-1560 (2016).
[3] Sala, F., Castriotta, M., Paiè, P., Farina, A., D’Annunzio, S., Zippo, A., Osellame R., Bragheri F., Bassi, A. (2020). High-throughput 3D imaging of single cells with light-sheet fluorescence microscopy on chip. Biomedical optics express, 11(8), 4397-4407.
Bio:
Andrea Bassi received his Ph.D. in Physics at Politecnico di Milano in 2006. He conducted research at Beckman Laser Institute, University of California (Irvine) in 2005/2006 as Research Specialist, at Politecnico di Milano from 2009 to 2014 as Researcher, at Max Planck Institute of Molecular Cell Biology and Genetics (Dresden) in 2013/2014 as Marie Curie Fellow. Currently he is an Associate Professor at the Department of Physics of Politecnico di Milano.
Hour: From 12:00h to 13:00h
Place: Online (Zoom)
L4H SEMINAR: Light Sheet Fluorescence Microscope with an Optofluidic chip
Abstract:
Opto-fluidic technologies integrate multiple fluidics and photonics devices in a miniaturized chip. Among many lab-on-chip applications, they can be used for optical microscopy [1].
The seminar will discuss the design and applications of a millimeter-scaled optofluidic device that incorporates light-sheet illumination and automatic sample delivery and scanning. This device upgrades a standard inverted microscope to a high throughput, three-dimensional, Light Sheet Fluorescence Microscope. A cylindrical lens fabricated by Femtosecond Laser Irradiation followed by Chemical Etching (FLICE) is integrated with an optical waveguide or an optical fiber on a fused silica chip. The lens is used to illuminate a single plane of the sample, at two alternating wavelengths. The samples flow in a three-dimensional fluidic network (fabricated by FLICE too) and is then imaged on a CMOS running at camera at 400kHz.
Several applications will be discussed in the seminar, together with the technological solutions for automatic sample alignment, including automatic imaging of tumor spheroids [2], Drosophila embryos, and high NA (1.1) imaging of cancer cells [3].
[1] Paiè, P., Martínez Vázquez, R., Osellame, R., Bragheri, F., and Bassi, A. “Microfluidic based optical microscopes on Chip” Cytometry Part A, 93, 987-996 (2018).
[2] Paiè, P., Bragheri, F., Bassi, A., and Osellame, R. “Selective plane illumination microscopy on a chip” Lab on a chip, 16(9), 1556-1560 (2016).
[3] Sala, F., Castriotta, M., Paiè, P., Farina, A., D’Annunzio, S., Zippo, A., Osellame R., Bragheri F., Bassi, A. (2020). High-throughput 3D imaging of single cells with light-sheet fluorescence microscopy on chip. Biomedical optics express, 11(8), 4397-4407.
Bio:
Andrea Bassi received his Ph.D. in Physics at Politecnico di Milano in 2006. He conducted research at Beckman Laser Institute, University of California (Irvine) in 2005/2006 as Research Specialist, at Politecnico di Milano from 2009 to 2014 as Researcher, at Max Planck Institute of Molecular Cell Biology and Genetics (Dresden) in 2013/2014 as Marie Curie Fellow. Currently he is an Associate Professor at the Department of Physics of Politecnico di Milano.
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Place: Online (Zoom)
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