ICFO - Group Details

SLN - Team Wieser

Dr. Stefan Wieser

Activities:

Our team works at the interface of physics and biology. We are developing live cell super-resolution imaging techniques for 3D imaging of whole cell dynamics. We mainly focus on immune cells with the aim to study the intrinsic coupling between leucocyte migration, cell polarization and antigen presentation. To gain a mechanistic understanding of these processes we apply advanced imaging techniques - with focus on SIM - and data analysis tools that allow for integrating molecular dynamics with large-scale cell behavior. The ultimate goal is to develop mathematical models with predictive capacity from the molecular scale to global cell behavior and immune cell function.

Lattice Light Sheet Microscopy
High NA-TIRF Structured Illumination Microscopy
2D/3D live cell single molecule imaging
Advanced biomimetic interfaces
Quantitative Data Analysis Tools and Monte Carlo Simulations
Immune cell migration
Temperature control of immune cell responses

SLN Research Facility homepage

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SLN - Team Wieser

Dr. Stefan Wieser

Dr. Stefan Wieser

Loïc Reymond

Boris Clement Karamata, Research engineer
Johannes Ziegler, Research engineer

SLN - Team Wieser

Dr. Stefan Wieser

Cooperative epithelial phagocytosis enables error correction in the early embryo E. Hoijman, H. Häkkinen, Q. Tolosa-Ramon, S. Jiménez-Delgado, C. Wyatt, M. Miret-Cuesta, M. Irimia, A. Callan-Jones, S. Wieser, V. Ruprecht Nature, (2021)
Mesoscale modeling and single-nucleosome tracking reveal remodeling of clutch folding and dynamics in stem cell differentiation P. A. Gómez-García, S. Portillo-Ledesma, M. V. Neguembor, M. Pesaresi, W. Oweis, T. Rohrlich, S. Wieser, E. Meshorer, T. Schlick, M. P. Cosma, M. Lakadamyali Cell Reports 34, 108614 (2021)
Fluorescence microscopy-based quantitation of GLUT4 translocation: High throughput or high content? V. Stadlbauer, P. Lanzerstorfer, C. Neuhauser, F. Weber, F. Stübl, P. Weber, M. Wagner, B. Plochberger, S. Wieser, H. Schneckenburger, J. Weghuber IJMS 21, 7964 (2020)
The nucleus measures shape changes for cellular proprioception to control dynamic cell behavior V. Venturini, F. Pezzano, F. Català Castro, H.-M. Häkkinen, S. Jiménez-Delgado, M. Colomer-Rosell, M. Marro, Q. Tolosa-Ramon, S. Paz-López, M. A. Valverde, J. Weghuber, P. Loza-Alvarez, M. Krieg, S. Wieser, V. Ruprech Science 370, eaba2644 (2020) Highlighted in Science
Modulation-enhanced localization microscopy (meLM) L. Reymond, T. Huser, V. Ruprecht, S. Wieser J. Phys. Photonics 2,(2020)
Cortical anchoring of the microtubule cytoskeleton is essential for neuron polarity L. He, R. Kooistra, R. Das, E. Oudejans, E. van Leen, J. Ziegler, S. Portegies, B. de Haan, A. van Regteren Altena, R. Stucchi, A. M. Altelaar, S. Wieser, M. Krieg, C. C. Hoogenraad, M. Harterink eLife 9, e55111 (2020)
A reconstituted mammalian APC-kinesin complex selectively transports defined packages of axonal mRNAs S. Baumann, A. Komissarov, M. Gili, V. Ruprecht, S. Wieser, S. P. Maurer Sci. Adv. 6, eaaz1588 (2020)
Hypolipidemic effects of herbal extracts by reduction of adipocyte differentiation, intracellular neutral lipid content, lipolysis, fatty acid exchange and lipid droplet motility R. Haselgrübler, P. Lanzerstorfer, C. Röhrl, F. Stübl, J. Schurr, B. Schwarzinger, C. Schwarzinger, M. Brameshuber, S. Wieser, S. M. Winkler, J. Weghuber Sci. Rep. 9, 10492 (2019)
SIMPLE: Structured illumination based point localization estimator with enhanced precision L. Reymond, J. Ziegler, C. Knapp, F. Wang, T. Huser, V. Ruprecht, S. Wieser Opt. Express 27, 24578 (2019)
Neuronal stretch reception – Making sense of the mechanosense R. Das, S. Wieser, M. Krieg Experimental Cell Research 378, 104-112 (2019)

How do cells sense physical forces? Cellular mechanosensing and motility in biomimetic 3D environments V. Venturini, F. Pezzano, M. Marro, P., Loza-Álvarez, M. A. de Castro, S. Wieser, V. Ruprecht Joint 12th EBSA European Biophysics Congress / 10th IUPAP International Conference on Biological Physics (ICBP), Madrid, Spain, July 2019
Mechanosensitive actomyosin network dynamics and migration plasticity in 3D environments V. Venturini, F. Pezzano, S. Wieser. V. Ruprescht Joint 12th EBSA European Biophysics Congress / 10th IUPAP International Conference on Biological Physics (ICBP), Madrid, Spain, July 2019
SIMPLE: Structured illumination localization microscopy S. Wieser, L. Reymond, J. Ziegler, V. Ruprecht, V. Ruprecht Joint 12th EBSA European Biophysics Congress / 10th IUPAP International Conference on Biological Physics (ICBP), Madrid, Spain, July 2019

High temperature triggers immediate morphological changes and a sustained increased efficiency in neutrophil migration I. Company-Garrido
Towards a size estimation of nanostructrues using structured illumination microscopy C. Kumar

Development and application of localization-based microscopy methods to study the structure and dynamics of chromatin through the process of cellular differentiation P. Gómez García

SLN - Team Wieser

Dr. Stefan Wieser

2021-08-30 Laserlab-Europe Position Paper New position paper details Laserlab’s missions as they align with Horizon Europe to solve important societal challenges
2020-10-16 When feeling the pinch, nuclei instigate cells to escape crowded spaces Scientists from CRG, ICFO, FH OÖ and UPF report on this new finding in a study published in the journal Science.
2020-04-24 Congratulations to New ICFO PhD graduate Dr. Pablo Gómez García graduated with a thesis entitled “Development and Application of Localization-Based Microscopy Methods to Study the Structure and Dynamics of Chromatin through the Process of Cellular Differentiation”
2016-11-22 New Team Leader Dr. Stefan Wieser joins ICFO as a PI Staff Scientist within the SLN Program