Neurophotonics and Mechanical Systems Biology

Our main research goal is to understand the importance of cell mechanical properties for health and disease on the molecular and systems level. Mechanical forces are ubiquitous signals that provide information about our environments and our own body during touch and as we walk, breathe, and fall in love. Although failures to sense and cope with mechanical forces are linked to human diseases including peripheral neuropathies and neurodegenerative disorders, little is known about the connections between biomechanics and disease. One reason for this gap is the technical challenge of detecting forces and the deformations resulting from them within a living cell or organism. To fill this gap and advance our understanding, we develop and deploy new optogenetic tools (FRET, synthetic biology) in vivo and optical tweezers in vitro to measure piconewton force and their consequences inside cells. From these data and informed by theoretical predictions, we derive insight and mechanistic understanding of how changes in protein and cell mechanics contribute to pathological transformations in mechanosensation and protection.

Due to the wealth of genetic tools available for it, we use the small round worm, Caenorhabditis elegans, with its compact nervous system consisting of only 302 neurons, its short lifespan and simple body plan, as a model. We exploit microfluidic and nanotechnological tools to apply precise forces to single cells or animals with the aim to visualize mechanical forces and their consequences as the animals crawl, feel and become old. In addition, we strive to assemble synthetic and synesthetic circuit based on second generation optogenetic tools. To achieve these goals, we built dedicated imaging systems for photon starved samples and precision illumination of single synapses in vivo.

We are hiring: PhD-students in Neuronal Cell Biology. Please contact Prof. Dr. Michael Krieg for further information.