Leica TCS SP5 STED CW
The Leica TCS SP5 STED CW system is based on the Leica TCS SP5 confocal system. It includes the STED CW module. The HyD detectors operate in photon counting mode providing high sensitivity and detection capabilities.
Resonant scanners provide fast scanning capabilities up to videorate at 512×512 pixels. The system is coupled to a MIRA 900F Ti:Sapp. fs laser source for multi photon imaging. Live imaging is possible due to controlled environmental conditions.
Techniques
STED CW, Confocal, hyper spectral (5D) laser scanning confocal microscopy, TPEF
Microscope objectives
10x, 20x, 40x, 63x and 100x
Excitation wavelengths
- STED laser: 592nm
- Argon laser: 458, 476, 488, 496, 514 nm
- HeNe laser: 543, 633 nm
- Ti:Sapphire laser: 750-850nm, pulses duration: 120fs, repetition rate 76MHz
Detectors
- 2 Backwards HyD’s
- 1 Forward PMT
- 3 Backwards PMT’s
- 2 Backwards APD’s
Scanning mirrors
- Galvanometric
- Resonant