Multimodal Nikon microscope
This is a multimodal workstation built on top of a commercial inverted microscope. This system contains an external scanning head where different femtosecond lasers are coupled.
This allows the simultaneous implementation of multiphoton imaging techniques such as TPEF, SHG, PSHG, THG, for each excitation laser.
Techniques
WF, TPEF, SHG, PSHG, THG, Femtosecod nanosurgery.
Microscope objectives
- for confocal/multiphoton: 2x, 10x, 20x, 40x, 60x and 100x
- specialized for multiphoton (water inmmersion): Olympus 25x , 1.05 NA and Nikon 25x, 1.10 NA
Excitation wavelengths
- Diode lasers 405, 561, 635nm
- Ti:sapphire 750-850nm
- Femtosecond pulsed fiber laser 1030nm
- Femtosecond pulsed fiber laser 1550nm
Detectors
- 1 Forward multiphoton PMT, Large area IR Photodiode, Vis. photodiode
- 2 Backwards multiphoton PMT’s
Scanning mirrors
Galvanometric